FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511823
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTAT18363612.14666002567761TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGTAT25840.17091947933058302TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGAT19740.13057084063412186TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTGGTAT15330.10140075921586059TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC193000.068.0618447
CGTATGC191550.068.0471546
TATGCCG191600.067.96081548
TCGTATG191650.067.8655345
ATGCCGT190400.067.6905449
CTCGTAT195600.067.42554544
TGCCGTC189950.067.300350
CTGCTTG190900.067.0776259
GCCGTCT188300.067.0534851
TGCTTGA193100.066.96158660
TCTCGTA197650.066.9564343
CCGTCTT192950.066.4897552
ATCTCGT199650.066.4785342
AATCTCG201450.066.3318941
GCTTGAA196250.066.15433561
TCTGCTT195700.066.1256258
CTTCTGC198500.065.24361456
TTCTGCT196750.064.9009857
GAATCTC206900.064.5972640
GAGAATC215250.064.1887538
Produced by FastQC (version 0.11.5)