FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580374
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCTCGTAT1468839.294192387371597TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC157400.068.0403447
TATGCCG157600.067.9360948
CGTATGC157800.067.8456946
TCGTATG158450.067.5452945
ATGCCGT158750.067.46649
TGCCGTC160100.066.8837150
CTCGTAT159650.066.8360644
CTGCTTG160500.066.78245559
GCCGTCT160550.066.72227551
TCTCGTA160650.066.33707443
ATCTCGT160600.066.3120542
CCGTCTT161750.066.2705652
TGCTTGA162700.065.8343360
CATCTCG161700.065.49300441
GCATCTC161050.065.1726740
TGCATCT161100.065.1110539
TTGCATC161400.064.9466638
GCTTGAA165450.064.75712661
TCTGCTT165800.064.62247558
ATTGCAT164300.064.0772437
Produced by FastQC (version 0.11.5)