FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187597
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGAAGATCTCGTAT2009039.183729910033705TruSeq Adapter, Index 9 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC217150.068.13412547
CGTATGC217300.068.0678846
TATGCCG217700.067.9298448
TCGTATG218700.067.6946145
ATGCCGT218500.067.6697549
CTCGTAT219300.067.2365544
TGCCGTC220800.066.9346850
CTGCTTG221450.066.7555459
TGCTTGA222000.066.56982460
GCCGTCT222100.066.5113751
GATCTCG221450.066.2375941
TCTCGTA223350.066.00168643
ATCTCGT223000.065.978242
CCGTCTT225300.065.613352
AGATCTC223500.065.5159240
GCTTGAA226500.065.262761
TCTGCTT227050.065.10609458
AAGATCT227050.064.338939
CTTCTGC230300.064.2799856
TTCTGCT232150.063.69234557
Produced by FastQC (version 0.11.5)