FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844353
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTAT20709711.228707302777723TruSeq Adapter, Index 10 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC218000.068.1342947
TATGCCG218100.068.02650548
CGTATGC218450.067.9760746
TCGTATG219000.067.6934945
ATGCCGT218850.067.69060549
CTCGTAT220100.067.4365144
TGCCGTC220650.066.9516650
GCCGTCT220400.066.7648351
TCTCGTA222600.066.7420343
CTGCTTG220300.066.6907459
CCGTCTT222050.066.4106252
ATCTCGT223850.066.398842
TGCTTGA222300.066.3669860
AATCTCG223950.066.3517341
GCTTGAA225300.065.7007761
TCTGCTT224350.065.6896758
CTTCTGC226150.065.139456
GAATCTC230300.064.63853540
CGAGAAT232550.064.2174737
TTCTGCT230000.063.83766657
Produced by FastQC (version 0.11.5)