FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730654
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTATCATCTCGTAT17750710.256642864489377TruSeq Adapter, Index 5 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG193400.068.0582948
CGTATGC193700.067.9870546
GTATGCC193700.067.9509247
TCGTATG194400.067.7242445
ATGCCGT194700.067.54172549
TGCCGTC195700.067.1985450
CTCGTAT195650.067.0609644
CTGCTTG196700.066.8963659
GCCGTCT196850.066.8473351
TGCTTGA197350.066.74119660
CCGTCTT198250.066.3595252
TCTCGTA197550.066.34511643
TCTGCTT199950.065.8284358
ATCTCGT199150.065.7769542
GCTTGAA201700.065.3538761
GTATCAT198250.065.2672837
CATCTCG200900.065.0123541
CTTCTGC204650.064.3850456
AGTATCA201800.064.17300436
TCATCTC203000.064.014140
Produced by FastQC (version 0.11.5)