FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984832
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCTCGTAT23422411.8006964821204TruSeq Adapter, Index 3 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC246950.067.9894347
CGTATGC247150.067.9309946
TATGCCG247550.067.8687748
TCGTATG248050.067.5716345
ATGCCGT249300.067.3587249
CTGCTTG248900.067.29310659
CTCGTAT248550.067.2791244
GCCGTCT250300.067.03873451
TGCCGTC250400.067.0242550
TGCTTGA250400.066.9285660
TCTCGTA251150.066.5129443
CCGTCTT252250.066.5049452
GCTTGAA252700.066.3055461
ATCTCGT252650.066.0748242
TCTGCTT254900.065.76404658
CATCTCG255100.065.2070141
CTTCTGC257500.065.1712856
TTCTGCT258450.064.8910857
CCATCTC256350.064.7356240
TCCATCT257850.064.0875939
Produced by FastQC (version 0.11.5)