FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945596
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTATCATCTCGTAT21450411.025104903587383TruSeq Adapter, Index 13 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC234300.068.0899247
CGTATGC234250.068.0728246
TCGTATG234450.067.92518645
TATGCCG234900.067.8502248
ATGCCGT235600.067.6057949
CTCGTAT236200.067.3033844
CTGCTTG237500.067.0779659
GCCGTCT238100.066.958251
TGCCGTC237950.066.9562650
TCTCGTA238350.066.63582643
TGCTTGA239350.066.5948160
CCGTCTT240550.066.2180252
ATCTCGT240250.066.1088442
CATCTCG240450.065.9357241
TCTGCTT242350.065.7933458
GCTTGAA242550.065.73064461
CTATCAT241200.065.2049837
GCCTATC242300.064.8961935
CTTCTGC246050.064.8340956
TTCTGCT246250.064.7655657
Produced by FastQC (version 0.11.5)