FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491423
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTAT17248011.564794159671669TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG183050.068.0887448
CGTATGC182900.068.082646
GTATGCC183200.068.01388547
ATGCCGT183800.067.7796349
TCGTATG184500.067.5468345
CTGCTTG185350.067.27933559
CTCGTAT184850.067.24853544
TGCCGTC185750.067.0748350
GCCGTCT186100.066.8756851
CCTATCT185000.066.6309639
CCGTCTT187100.066.5556752
TGCTTGA187550.066.46924660
CTATCTC186050.066.34675640
TCTCGTA187850.066.1373143
TATCTCG186950.066.11874441
GACCTAT187450.065.7853837
ATCTCGT188900.065.76968442
TCTGCTT189600.065.75938458
GCTTGAA190200.065.48794661
ACCTATC188100.065.4255938
Produced by FastQC (version 0.11.5)