FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752216
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCCAGATCTCGTAT19976011.400420952667936TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG215050.068.0433648
GTATGCC215000.068.0409647
CGTATGC215650.067.8826346
ATGCCGT216100.067.7347449
TCGTATG216700.067.57178545
TGCCGTC217500.067.3064350
CTCGTAT217100.067.2860944
CTGCTTG218550.066.9491259
GCCGTCT218850.066.8969751
TGCTTGA220050.066.54047460
TCTCGTA219900.066.4293343
CCGTCTT220350.066.425752
TCTGCTT221700.066.0452458
ATCTCGT221250.065.9923642
GCTTGAA222100.065.9893461
GATCTCG222000.065.5978741
AGATCTC221950.065.439240
CAGATCT222700.065.0616639
CTTCTGC227000.064.5876956
TTCTGCT227150.064.448957
Produced by FastQC (version 0.11.5)