FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154133
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGAGCTATCTCGTAT39142118.1706979095534TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC419300.068.2879347
CGTATGC419700.068.25461646
TATGCCG419500.068.2184148
TCGTATG420350.068.1042845
ATGCCGT420250.068.0946649
TGCCGTC422050.067.82555450
CTCGTAT422200.067.68152644
CTGCTTG422750.067.6568559
GCCGTCT423200.067.62627451
TGCTTGA423400.067.5549860
TCTCGTA423450.067.43214443
GCTATCT420800.067.2117339
CCGTCTT426100.067.2070952
TATCTCG423050.067.1551741
ATCTCGT425650.067.1051742
GCTTGAA427000.067.01002561
CTATCTC424100.066.85531640
TCTGCTT428850.066.7026658
AGCTATC424050.066.63367538
GAGCTAT424750.066.5037837
Produced by FastQC (version 0.11.5)