FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128321
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATCTCGTAT30327814.249636215589659TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG321900.068.2237348
GTATGCC322450.068.1383347
CGTATGC322850.068.0382746
ATGCCGT323100.067.931849
TCGTATG323850.067.8590145
TGCCGTC325050.067.5350450
CTCGTAT323950.067.48469544
CTGCTTG325250.067.4811759
GCCGTCT326150.067.30727451
TGCTTGA327700.067.0740160
CCGTCTT328050.066.94034652
TCTCGTA326150.066.90987443
ATCTCGT327000.066.7129742
AATCTCG326650.066.6360241
GCTTGAA330250.066.5561161
TCTGCTT330400.066.44147558
CAATCTC326750.066.4379940
TCAATCT328200.065.9205339
CTTCTGC334650.065.63644456
TTCTGCT336350.065.26613657
Produced by FastQC (version 0.11.5)