FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412639
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTAT38965416.150530601552905TruSeq Adapter, Index 7 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC413900.068.4117447
CGTATGC414250.068.3595646
TATGCCG414400.068.3320348
ATGCCGT415200.068.1863449
TCGTATG415500.068.17074645
TGCCGTC416900.067.9027150
CTCGTAT416950.067.82455444
CTGCTTG419100.067.5504259
GCCGTCT419200.067.5329651
TGCTTGA420750.067.3256660
CGTATCT415050.067.2805639
TCTCGTA420300.067.2006843
CCGTCTT421750.067.1260352
GTATCTC417550.067.0788940
TATCTCG419300.066.926941
ATCTCGT421950.066.91302542
TCTGCTT424150.066.7722958
GCTTGAA424650.066.7567961
CCGTATC417900.066.7309838
GACCGTA417600.066.57629436
Produced by FastQC (version 0.11.5)