FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077026
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTCTATCTCGTAT29732614.314986909167244TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG317200.068.2313148
CGTATGC317350.068.1577646
GTATGCC317950.068.06544547
ATGCCGT318450.067.9386949
TCGTATG318500.067.8424645
CTCGTAT319300.067.54095544
TGCCGTC320700.067.4402150
CTGCTTG321500.067.30784659
GCCGTCT321550.067.2401651
TGCTTGA322550.067.10074660
CCGTCTT322950.066.9920352
TCTCGTA322350.066.80418443
TATCTCG322200.066.5713941
GTCTATC320250.066.5553638
GCTTGAA325600.066.5060461
GGTCTAT320350.066.5018137
TCTGCTT325800.066.4409958
ATCTCGT324500.066.3923342
CTATCTC322450.066.3461240
CGGTCTA322150.066.1676136
Produced by FastQC (version 0.11.5)