FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484836
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAGGTATCTCGTAT27596311.10588384907495TruSeq Adapter, Index 18 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG288050.068.2742548
CGTATGC288500.068.2256746
GTATGCC289000.068.13322447
TCGTATG289750.067.9568645
ATGCCGT290100.067.8052249
CTCGTAT291350.067.4769144
TGCCGTC292700.067.1591450
CTGCTTG293100.067.0794359
GCCGTCT293000.067.0678451
GGTATCT291350.066.86021439
TGCTTGA294500.066.8172860
AGGTATC291800.066.6718138
TCTCGTA295550.066.4943243
GTATCTC293800.066.4575240
CCGTCTT296350.066.3582752
TCTGCTT297400.066.1344258
CAGGTAT294250.066.12237
TATCTCG296200.066.0648241
ATCTCGT297650.066.0251942
GCTTGAA299500.065.72517461
Produced by FastQC (version 0.11.5)