FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114377
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTAT31120527.926366032321198TruSeq Adapter, Index 16 (97% over 38bp)
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT16800.15075688030172912No Hit
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT14150.12697677715889685No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATATCGTAT12450.11172161665217427TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG330250.068.6592948
GTATGCC330300.068.6502547
ATGCCGT330350.068.6415949
CGTATGC330400.068.60828446
TGCCGTC330700.068.5402550
TCGTATG331050.068.4735845
GCCGTCT331500.068.37485551
CTGCTTG331300.068.35409559
CTCGTAT331450.068.16170544
CCGTCTT332800.068.1498452
TGCTTGA332550.068.1362160
TCTGCTT333150.067.9955358
TCTCGTA332250.067.9449143
GCTTGAA334550.067.7811861
AATCTCG332350.067.75597441
ATCTCGT333250.067.7515342
TTCTGCT335450.067.5323557
CTTCTGC335650.067.4803456
CGTCTTC337500.067.1726853
CTTGAAA339350.066.8284462
Produced by FastQC (version 0.11.5)