FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246322
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT2038949.07679308665454TruSeq Adapter, Index 4 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG228450.068.0857548
GTATGCC228550.068.05443647
CGTATGC228450.068.0199146
ATGCCGT229150.067.88531549
TCGTATG228600.067.8681245
TGCCGTC231550.067.2164150
CTCGTAT231350.067.06138644
CTGCTTG231750.066.90459459
TGCTTGA232200.066.888160
GCCGTCT232950.066.7252651
CCGTCTT234100.066.4139152
TCTCGTA235050.065.9596143
GCTTGAA236250.065.83034561
TCTGCTT236350.065.669258
CGACATC234000.065.6602638
ATCTCGT236200.065.6251142
CATCTCG235950.065.4439541
TACCGAC234100.065.37475635
GACATCT236000.065.22139
ACCGACA235450.065.1917436
Produced by FastQC (version 0.11.5)