FastQCFastQC Report
Fri 8 Sep 2023
HMNWVBGXT_n01_HW10_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNWVBGXT_n01_HW10_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14615477
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1986331.3590593040514516TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT376440.2575625824596761TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTTT214700.14689907144323788TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)