FastQCFastQC Report
Fri 8 Sep 2023
HMNWVBGXT_n01_DO68_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNWVBGXT_n01_DO68_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9473141
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG204370.21573625896627105TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTGG157900.1666817795702608TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC128160.13528775724968098TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph