FastQCFastQC Report
Fri 8 Sep 2023
HMNWVBGXT_n01_DO66_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNWVBGXT_n01_DO66_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26337029
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC677660.2573031301290665TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC372790.1415459579742271TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC335180.1272656836122252TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)