FastQCFastQC Report
Sat 20 Oct 2018
HMNKTBGX7_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNKTBGX7_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500672595
Sequences flagged as poor quality0
Sequence length99
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG31334530.6258487145676508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26078260.5208645382318159No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG8936130.17848250711625227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA7581300.15142230822519856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG14832650.063.4272082
AAGCAGT15251000.062.0127071
CAGTGGT16323900.057.3789984
AGTGGTA17147450.054.7975125
GCAGTGG17419100.053.989853
GTGGTAT18749900.050.127216
GGTATCA19012600.049.3113178
TGGTATC19491650.048.076267
GTATCAA27222300.034.4049389
AGAGTAC18382650.029.02002318-19
CATGGGG13824650.028.97307824-25
GCAGAGT18718000.028.55264316-17
TACATGG19036850.027.81643122-23
CAACGCA19761600.026.9485612-13
ACGCAGA20090150.026.5489114-15
TGGGGGC7637100.026.24605226-27
ATCAACG20441000.026.16405310-11
CCTCACT7316800.026.11051272-73
CGTTCCT7433000.025.81542668-69
TGCCTCA7833450.025.52097140-41
Produced by FastQC (version 0.11.5)