FastQCFastQC Report
Sat 20 Oct 2018
HMNKTBGX7_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNKTBGX7_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500672595
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA1537650.018.532621
GTCTGAC1869800.018.4782687
TCTGACC2084650.018.3360588
CTGACCT2098200.018.1960939
CAATGGA1547850.018.1833368
CCGGGAT1431600.017.9642331
GGGGGGC602700.017.8546621
TGGGCGT1323800.017.8204731
GATGAAA1550700.017.7867261
TGACGGC1252650.017.7829151
TGACCTC2151850.017.7361210
GCGCGAT1023950.017.5871961
ATGGGAG1348100.017.5389861
TGCTACC1890100.017.4606971
GGGACCT963850.017.4415821
CGCTGGA1278800.017.3617821
ATAACGC1292800.017.3411251
GGCGACT1339700.017.3094921
GACTAAC1143400.017.2912451
CGTGAGC1156250.017.2834761
Produced by FastQC (version 0.11.5)