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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

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        Report generated on 2017-06-21, 06:06 based on data in: /mnt/gencore/sites/core-fastqc.bio.nyu.edu/html/HMNGTBGX2/merged


        General Statistics

        Showing 26/26 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HMNGTBGX2_n01_jes_009
        44.7%
        45%
        12.0
        HMNGTBGX2_n01_jes_010
        39.9%
        45%
        10.1
        HMNGTBGX2_n01_jes_117
        46.5%
        45%
        13.9
        HMNGTBGX2_n01_jes_119
        50.9%
        45%
        18.7
        HMNGTBGX2_n01_jes_120
        41.1%
        45%
        9.7
        HMNGTBGX2_n01_jes_130
        86.5%
        44%
        12.5
        HMNGTBGX2_n01_jes_131
        44.7%
        45%
        12.6
        HMNGTBGX2_n01_jes_132
        45.9%
        45%
        13.3
        HMNGTBGX2_n01_shrt_02g
        68.3%
        44%
        50.5
        HMNGTBGX2_n01_shrt_03g
        74.2%
        45%
        90.3
        HMNGTBGX2_n01_shrt_04g
        58.6%
        45%
        15.7
        HMNGTBGX2_n01_shrt_05g
        57.5%
        45%
        11.0
        HMNGTBGX2_n01_shrt_07g
        58.3%
        44%
        24.7
        HMNGTBGX2_n01_shrt_08g
        60.2%
        45%
        23.6
        HMNGTBGX2_n01_shrt_09g
        55.5%
        45%
        12.0
        HMNGTBGX2_n01_shrt_09r
        65.2%
        46%
        13.0
        HMNGTBGX2_n01_shrt_10g
        62.3%
        45%
        13.5
        HMNGTBGX2_n01_shrt_10r
        85.7%
        45%
        12.9
        HMNGTBGX2_n01_shrt_11r
        55.7%
        45%
        13.1
        HMNGTBGX2_n01_shrt_12g
        50.5%
        44%
        11.9
        HMNGTBGX2_n01_shrt_12r
        54.8%
        45%
        12.8
        HMNGTBGX2_n01_shrt_13g
        47.8%
        45%
        8.5
        HMNGTBGX2_n01_shrt_13r
        61.4%
        46%
        18.3
        HMNGTBGX2_n01_shrt_14g
        58.5%
        45%
        12.0
        HMNGTBGX2_n01_shrt_14r
        66.9%
        46%
        13.4
        HMNGTBGX2_n01_shrt_15r
        71.8%
        45%
        19.3

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 3/3 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF ReadsUndetermined
        4.0
        526710674
        490758191
        0.6%

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 26/26 rows and 3/3 columns.
        LibraryTotal Read CountPortion (%)Perfect (%)
        shrt_13r
        18268626
        3.8
        98.9
        jes_010
        10111308
        2.1
        99.1
        jes_117
        13888921
        2.9
        98.8
        shrt_12r
        12787557
        2.7
        98.8
        shrt_10r
        12900265
        2.7
        98.9
        shrt_05g
        11029532
        2.3
        99.0
        jes_120
        9720950
        2.0
        98.9
        jes_132
        13250008
        2.8
        99.0
        shrt_09r
        12990706
        2.7
        99.0
        shrt_15r
        19331761
        4.0
        98.9
        shrt_09g
        12045736
        2.5
        98.8
        shrt_14r
        13355648
        2.8
        98.8
        jes_131
        12616010
        2.6
        98.9
        shrt_07g
        24679085
        5.1
        99.0
        jes_130
        12530105
        2.6
        99.0
        jes_009
        12045782
        2.5
        98.0
        shrt_11r
        13071178
        2.7
        99.1
        shrt_03g
        90286416
        18.8
        99.1
        shrt_04g
        15656877
        3.3
        98.8
        jes_119
        18652992
        3.9
        99.0
        shrt_02g
        50532694
        10.5
        99.1
        shrt_08g
        23639112
        4.9
        99.1
        shrt_10g
        13482829
        2.8
        99.0
        shrt_12g
        11926701
        2.5
        99.0
        shrt_13g
        8490997
        1.8
        98.7
        shrt_14g
        11994544
        2.5
        98.7

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (75bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        loading..

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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