FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_15r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_15r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19331761
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG252010.13036060191309007No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT233520.12079603094617196No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC197210.10201346892298119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCC121950.032.136716
TACTCTA86450.031.5656094
CTGTTAC131900.030.0619561
ACTCTAG95100.029.5290055
CTAGTTG90450.029.3289768
TACCCTA133150.029.2244958
TACTACC134850.029.0368735
TCTAGTT99950.028.0599487
GTTACTA144650.027.2128033
CTACCCT146450.026.7588867
GTACTCT102300.026.4053673
CTCTAGT105050.026.3708696
TTACTAC152350.025.9051574
TGTACTC107250.025.2190192
TTGTACT110400.024.2256051
CCTACAC163750.024.10043711
GTTACCT112800.023.36722614
TGTTACT170350.023.2492622
ACCTCTA113650.023.1020517
CTAATGC113800.022.91787521
Produced by FastQC (version 0.11.2)