FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_14r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_14r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13355648
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT325160.2434625410912297No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC266810.19977315964002645No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC231180.1730953076930449No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG192100.14383427895074802No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT172240.12896416557249787No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT142680.10683120729147698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG118150.036.034975
GCTGACT56500.035.4904821
TACTCTA64850.034.4750184
CTAGTTG65900.033.1890268
ACTACCC91950.033.094916
CTGTTAC94200.032.4980241
TCTAGTT75950.030.2053997
TACTACC100300.030.1332445
TACCCTA100350.030.0458688
CTCTAGT76100.029.696216
GTTACTA103250.029.5059853
TGTACTC75450.029.3115162
TTGTACT75900.028.6927261
CTACCCT108200.027.8978987
TTACTAC110300.027.5575034
GTACTCT80600.027.5242443
TAGTTGT80500.027.1267999
GACTCTA81450.026.0933074
TGTTACT117450.025.9680162
GTTACCT82950.025.86985214