FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_14g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_14g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11994544
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG125030.10423906069292838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCC68000.032.2660336
ACTCTAG80100.031.3110395
TACTCTA54950.031.0764394
CTAGTTG59300.028.9704868
TACCCTA75450.028.7589178
TACTACC78400.027.985735
CTGTTAC79950.027.4493471
CTACCCT79050.027.3619337
TCTAGTT67300.026.7056877
GTTACTA82450.026.4853083
CTCTAGT68450.025.653216
GTACTCT66600.025.4848963
GTTACCT66650.025.0529514
CTAATGC66800.024.78938721
GGACACC63850.023.9370729
CCTACAC93350.023.7985711
TTACTAC94250.023.3158824
ACCTCTA74150.022.98535517
TGTTACC73850.022.8896613
TACCTCT73800.022.67352316
Produced by FastQC (version 0.11.2)