FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_13r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_13r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18268626
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT509050.27864711883641385No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC454520.24879813074064794No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC431320.23609876298305082No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT316160.17306172888973698No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT249180.13639777835508812No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTG226620.12404873798390749No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT217090.11883214424554972No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC216650.1185912941673884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT85600.037.9778251
ACTCTAG125000.029.2012465
GACTCTA120450.028.6715154
CTGACTC123650.026.450872
CTAGCAG128000.025.3877988
TGACTCT154200.021.9710923
CTCTAGC160200.021.212876
TAGCAGA166700.019.9905919
TCTAGCA377550.017.7808977
GTGCGCA201950.017.74952568
GGTGCGC204850.017.49820167
TGCGCAT206750.017.4041969
AGGTGCG218600.016.44459266
CTATCGA209500.015.64467317
TCTATCG211050.015.46434516
GCGCGCC54000.015.3329862
GCAGATC213800.015.15106611
AGCGCGC55200.014.99977761
AGCAGAT221050.014.76338510
TACCCTA23850.014.753538
Produced by FastQC (version 0.11.2)