FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_13g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_13g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8490997
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG129460.1524673722061143No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC117650.13855852263285454No Hit
AGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGAT92760.10924512162705982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT32250.025.790431
ACTCTAG47900.022.7598485
GACTCTA42400.021.073984
CTAGCAG45650.018.9680468
TACCCTA12500.017.6628048
CTGACTC49200.017.530232
ACTACCC15000.016.1000466
TCTAGCA121600.015.2346667
CTCTAGC58500.015.2154296
CGGGAAT62250.014.470131
TGACTCT63800.014.1675023
TAGCAGA61950.013.5874579
CTACCCT18000.013.0325277
GCAGATC81900.012.9320432
CTATCGA68850.012.4762788
CCTACAC21300.012.47098611
TCTATCG70750.012.38517
AGATCTA88050.012.1463284
GATCTAT79900.012.0468975
ATCTATC83050.011.6731226
Produced by FastQC (version 0.11.2)