FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_12r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_12r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12787557
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT217930.17042348276531633No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC196110.15336002021339964No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC183230.14328772884453223No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT134480.10516473162152866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT48550.029.855781
ACTCTAG72950.023.6470625
GACTCTA70250.022.2966844
CTAGCAG69250.021.321158
CTGACTC73350.020.225812
CTCTAGC93000.017.3618246
GTGCGCA87750.017.2203768
GGTGCGC89600.016.94195667
TGACTCT94600.016.5210573
TGCGCAT93800.016.22033169
TAGCAGA91800.016.0461229
TCTAGCA185150.015.6882687
AGGTGCG98550.015.47311366
GGGGGCC53400.014.79609339
GCGCGCC24700.014.5259162
AGGGGGC55650.014.19786738
CTATCGA107750.013.51224817
AGCGCGC27000.013.28867461
CAGGGGG60500.013.1737537
CGGGAAT81300.013.1170541
Produced by FastQC (version 0.11.2)