FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_12g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_12g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11926701
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG54150.020.1966295
GCTGACT38650.017.9473881
GACTCTA50800.015.4840224
CTAGCAG47600.015.36422358
TCTAGCA113200.013.7135417
TACCCTA19350.013.3709428
CTGACTC58100.012.7666452
CTAGTTG28650.012.7632988
ACTACCC22450.012.755096
CGGGAAT61450.012.6361681
CTCTAGC68850.011.5752346
TACTCTA33100.011.5693154
CTACCCT25750.010.9855077
TCTAGTT43100.010.965467
GGACACC26050.010.86031529
CTATCGA64800.010.80798217
GCCGAAT22100.010.77485751
TCTATCG66300.010.56341316
CTAGCAT77700.010.4778628
CGGCCCT6302.0008883E-1010.4080111
Produced by FastQC (version 0.11.2)