FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_11r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_11r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13071178
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT244030.1866931962826916No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC225830.17276943210474222No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC204730.15662704616217452No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT155070.1186350610480555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT51350.029.906191
ACTCTAG77050.022.5667925
GACTCTA74300.022.0088044
CTAGCAG75900.020.4531128
CTGACTC78150.019.9976732
GTGCGCA102300.017.6036868
CTCTAGC97050.017.2408286
GGTGCGC104700.017.2333767
TGCGCAT106900.016.94299569
TAGCAGA96550.016.3644689
TGACTCT101350.016.2708823
AGGTGCG113950.015.92508166
TCTAGCA195450.015.9206047
GGGGGCC63200.015.34119939
AGGGGGC68150.014.32816838
CAGGGGG70050.013.84102537
TCTATCG113000.013.80035316
CTATCGA112750.013.76985717
AAGGTGC134400.013.73271765
GCAGGGG72550.013.36407736
Produced by FastQC (version 0.11.2)