FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_10r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_10r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12900265
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG1653261.2815705723874666No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT1171730.9082991705984333No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACA881860.6835983601887248No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT877820.6804666415767429No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC871830.6758233261099675No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGAT591230.4583084145945839No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAAC560920.43481277322597633No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT552830.4285415842232698No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC535880.41540231925468196No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAG504670.39120901779924677No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAG457590.35471364347941686No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTG373040.28917235421132825No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTT355050.27522690425351726No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG342250.26530462746307926No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT341990.2651030812157735No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC332310.2575993593930047No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA320080.24811893399089088No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTGG303780.23548353464056745No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGG281910.218530394530655No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA279270.21648392494262714No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA277210.214887058521666No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCATAGGCTACTAGTGGCACACTGGGTTT276360.214228157328551No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC271350.21034451617854363No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGT259260.20097261567882518No Hit
GCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATC257990.19998813977852395No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTT255100.19774787572193284No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTC242060.1876395562416741No Hit
ATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCT214500.16627565402726222No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTA205890.15960137252994414No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAGCAGATAGTACCAAACC203690.15789598120658763No Hit
CACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC198950.15422163808262854No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAGCAGAT198320.15373327602184916No Hit
CCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCT187800.1455784047847079No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGG184790.14324511938320647No Hit
TTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAG183730.14242343083649833No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGT180580.13998162053260146No Hit
GGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTC173870.13478017699636402No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT169070.13105932319994976No Hit
CATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCA167870.1301291097508462No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCA166930.1294004425490484No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTAT166350.12895083938198168No Hit
ATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTGGTACT160730.12459433972867998No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCT154310.11961769777597592No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGA149500.11588909220081912No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAGCAGATAGTACC147880.11463330404452932No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGT137560.10663346838223867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT328150.060.230251
TGTACTC353950.056.3789062
CTGTTAC484050.055.2692341
TACTCTA371150.053.7196924
GTACTCT373200.053.4153633
ACTCTAG374850.053.1252255
CTCTAGT385050.051.566016
TCTAGTT390500.050.760527
TGTTACT531000.050.3609052
CTAGTTG428500.046.089948
TAGTTGT432200.045.647489
GTTACTA622450.042.9452823
AGTTGTT467950.042.22649410
GTTGTTA469800.042.0014711
TGTTACC469600.041.92548813
TTGTTAC475600.041.56259512
TTACTAC657000.040.7289054
GTTACCT490350.040.11129814
TACCTCT493950.039.8200416
TTACCTC506850.038.86738215
Produced by FastQC (version 0.11.2)