FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_10g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_10g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13482829
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG137010.10161813963523529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG90200.031.516425
CTAGTTG62900.031.3713058
TACTCTA63350.031.3683784
ACTACCC74200.030.1294026
TCTAGTT71650.028.503147
TACTACC82250.027.1384185
TACCCTA81800.027.0328568
GTACTCT74600.026.5453993
CTCTAGT77650.026.4803816
CTGTTAC85450.026.4534531
CTACCCT85150.026.0908647
GTTACTA88650.025.2181053
GTTACCT79800.024.03752114
GGACACC75100.023.888729
CTAATGC81350.023.32377221
ACCTCTA83750.023.15183417
TGTTACC85850.022.66361813
TACCTCT86350.022.65442516
TTACTAC98300.022.6371844
CCTACAC102900.022.29395111
Produced by FastQC (version 0.11.2)