FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_09g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_09g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12045736
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG73000.029.7265935
TACTCTA49000.027.2477384
CTAGTTG50550.025.7961888
ACTACCC52100.025.163156
TCTAGTT58400.024.041777
TACCCTA56700.023.059038
CTCTAGT60150.022.8852886
GTACTCT58200.022.7034233
GTTACCT58800.021.70963914
CTACCCT60900.021.3554617
GGACACC55200.020.93732829
TACTACC62450.020.7717345
CTGTTAC63700.020.5343631
GCTGACT40200.020.4330081
TGTTACC64900.020.14589713
CTAATGC62400.019.79236621
ACCTCTA64250.019.76146117
TACCTCT67200.019.3560416
CCTACAC71150.019.29722411
GACACCT60350.019.20787830
Produced by FastQC (version 0.11.2)