FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_08g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_08g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23639112
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG359470.1520657797974814No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC298060.12608764660872201No Hit
AGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGAT250790.10609112558881231No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG239180.10117977358878794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT87900.026.1104221
ACTCTAG130000.021.3899275
GACTCTA121050.020.6342034
CTAGCAG124400.018.884598
CTGACTC130650.017.9564232
TCTAGCA329450.016.2720537
CTCTAGC162500.015.3074616
TGACTCT170900.014.7366063
TAGCAGA169800.014.2619939
CGGGAAT170800.013.21511651
CTATCGA184200.012.60529817
CTAGCAT234750.012.57908258
TACCCTA26450.012.2597898
TCTATCG189000.012.08434116
TTCTAGC254800.012.0506886
ATTCTAG265800.011.7855125
GGACAGC247300.011.60583522
GATTCTA290700.011.3218464
GGTCGGC146400.011.24026465
CGATTCT298400.011.0182033
Produced by FastQC (version 0.11.2)