FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_07g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_07g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24679085
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT82600.021.0996531
ACTCTAG115200.020.9933035
GACTCTA107650.017.8506774
CTAGCAG100100.017.369288
TACCCTA36100.015.4807918
TCTAGCA240200.014.5629817
CTGACTC126950.013.4248512
CTCTAGC148600.013.1637466
CGGGAAT132850.012.7291241
CTATCGA139100.012.22779817
ACTACCC47600.011.9589286
TAGCAGA149550.011.9258539
TCTATCG146500.011.61012416
CTACCCT51650.011.3543947
CCTACAC64550.011.27642811
TGACTCT170800.011.0487453
CTAGTTG54700.011.0366198
CAGGGCG85400.010.4623028
AGGGCGG84800.010.3736049
CTAGCAT164950.010.3523338
Produced by FastQC (version 0.11.2)