FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_05g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_05g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11029532
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG137770.12491010497997558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA54700.034.24744
CTAGTTG57750.032.914648
ACTCTAG80500.032.871425
TCTAGTT64500.030.8606877
ACTACCC71250.029.8273626
CTCTAGT64900.029.6625526
GTACTCT65650.028.6402533
CTGTTAC76300.028.4956631
TACCCTA75800.027.807478
TACTACC77250.027.7339765
CTACCCT80400.026.3881267
GTTACTA81450.026.1765543
ACCTCTA73400.025.28803317
GTTACCT73800.025.1035414
CTAATGC74000.024.89481221
GGACACC68550.024.86236629
TTACTAC88550.024.3114624
TGTTACC77100.024.16188613
TACCTCT77850.023.93117316
CCTACAC91500.023.86558511
Produced by FastQC (version 0.11.2)