FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_04g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_04g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15656877
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG88650.025.6848645
ACTACCC46700.023.7877776
TACCCTA46800.023.2930078
GCTGACT54050.022.8585781
CTACCCT54000.020.4428737
TACTCTA48450.020.364934
TACTACC57550.019.2429565
GACTCTA74100.019.0421434
CCTACAC62300.018.82668511
CTAGTTG51550.018.469918
CTAGCAG68700.018.2277988
CTGACTC75650.017.238322
GGACACC50050.016.88859429
TCTAGTT64200.016.8188027
CTGTTAC72700.015.6653981
GACACCT54750.015.37578230
CTCTAGT62700.015.3515246
CTAATGC59050.015.13107321
GTACTCT63600.015.02573
GTTACCT60550.014.81391714
Produced by FastQC (version 0.11.2)