FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_03g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_03g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90286416
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG1312540.14537513594514592No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC1178490.1305279412132164No Hit
AGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGAT943590.10451073835957782No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG923920.10233211605165499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT344850.027.4706761
ACTCTAG489400.022.107225
GACTCTA470800.021.6101954
CTAGCAG477500.020.1491748
CTGACTC523600.018.4822772
TCTAGCA1259200.016.3773467
CTCTAGC630650.015.9686776
TGACTCT663950.015.4274953
TAGCAGA636150.015.3898799
CGGGAAT665200.013.8884361
CTATCGA715400.013.122251517
TCTATCG735550.012.8377816
CTAGCAT912250.011.8853678
ATCGCGC361800.011.87279358
TTCTAGC975300.011.5567086
CTTACAG649750.011.5275532
GATCTAT819700.011.43961814
ATTCTAG1004500.011.4165035
GCAGATC836200.011.36973811
TCGCGCT378250.011.32878259
Produced by FastQC (version 0.11.2)