FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_shrt_02g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_shrt_02g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50532694
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT165100.019.4401361
ACTCTAG224750.019.0035885
GACTCTA217950.016.6047214
CTAGCAG207700.015.89479358
TACCCTA70250.014.388018
TCTAGCA493250.014.2883057
CTGACTC246450.013.088692
CTCTAGC296200.012.218196
CGGGAAT285700.011.9588211
ACTACCC92300.011.5123886
TAGCAGA308600.010.798439
CTATCGA292550.010.7552317
TGACTCT343050.010.6802053
CCTACAC116650.010.58709711
ATCGCGC140950.010.47699658
TCTATCG302150.010.32214516
CTAGCAT351950.010.2623118
CTACCCT100300.010.2493147
ATTCTAG392450.010.1973745
AGGGCGG179650.09.965979
Produced by FastQC (version 0.11.2)