FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_jes_132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_jes_132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13250008
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23450.026.77361146
CGTATGC24250.025.46426644
GTATGCC26650.024.07681745
CGGGCGC10850.021.94093315
GCACACG37500.021.61789111
ACGTCTG40050.020.6749515
CACACGT39900.020.57733212
ATGCCGT30400.018.95052747
TCGTATG32800.018.72200843
ACACGTC45150.018.10834713
GCGGGCG13350.017.57324414
TAGCCCG14450.017.19090734
GGCGGGC14050.017.18739113
CTCGTAT37800.016.51997642
TCGGGCG16650.016.3678367
CACGTCT50200.016.3567714
GTCACAC47950.016.26082229
AGCACAC53650.015.56045910
CCGACTG34700.015.211022
ATCGGGC18050.014.9079226
Produced by FastQC (version 0.11.2)