FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_jes_131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_jes_131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12616010
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20150.023.96835146
CGTATGC20850.023.49475744
GTATGCC26300.019.01927845
ATGCCGT25950.018.34553347
GCACACG31400.017.90748211
TCGTATG29900.016.03765543
CACACGT35250.015.75615512
ACGTCTG36300.015.49215
ACACGTC39750.014.14606613
CTCGTAT34050.013.88101942
CGCGCGT15300.013.7593911
CGGGCGC8600.013.23859815
TGCCGTC37950.012.63522648
AGCACAC47450.012.57732310
GCCAGTA30850.012.5292091
CACGTCT45950.012.388185514
CGGGCAT18250.012.1025781
CGCGGGG6000.012.078941
GCGGGCG9600.011.85910814
CGCGTAT12600.011.7776521
Produced by FastQC (version 0.11.2)