FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_jes_130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_jes_130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12530105
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1811341.4455904399843418TruSeq Adapter, Index 6 (100% over 63bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243220.1941085090667636No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG230270.18377340014309537TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG288900.064.7311146
CGTATGC289200.064.6647444
GTCACGC293850.064.5041329
GTATGCC290700.064.3661545
ATGCCGT294600.063.4320947
TCACGCC299100.063.2219630
TCGTATG296600.063.20460543
CACGCCA299900.063.08806631
AGTCACG302100.062.84512728
CTCGTAT297950.062.32971242
CGCCAAT303050.062.20338433
TATCTCG299850.061.89614539
TGCCGTC302100.061.69769348
GCCAATA307200.061.3076534
ACGCCAA309600.061.2229132
ACGTCTG315100.060.93166415
GCCGTCT305000.060.73829349
GCACACG318150.060.50391811
CACACGT318350.060.4011312
TCTCGTA308150.060.32758341
Produced by FastQC (version 0.11.2)