FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_jes_120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_jes_120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9720950
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23050.040.40806246
CGTATGC23850.039.9211144
GTATGCC27950.033.81786745
ATGCCGT29600.031.81603847
TCGTATG30200.030.84236543
TAGGCAT32100.030.31158635
CACTTAG33400.029.74978331
ACTTAGG34050.029.38497532
TTAGGCA34600.028.7182934
ACGTCTG36350.028.56902115
CTCGTAT32650.027.26093542
GCACACG38650.027.22276511
CACACGT39400.026.87981612
ACACGTC40050.025.92688613
TGCCGTC37500.024.65363548
CTTAGGC42350.023.70691733
CACGTCT46250.022.67679814
GCCGTCT40700.022.37623249
TCTCGTA40150.022.34321441
TCACTTA46250.021.78255830
Produced by FastQC (version 0.11.2)