FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_jes_119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_jes_119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18652992
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC29400.026.8711344
TATGCCG31750.023.46908846
GTATGCC39250.020.12724545
TCGTATG42500.018.50757643
GCACACG46800.017.83834311
ATGCCGT43000.017.56959747
ACGTCTG50750.016.58691215
CACACGT53950.015.66618112
TATCTCG51650.014.83051239
CTCGTAT50400.014.78586242
CCGATGT56150.014.74612433
CGATGTA58000.014.63345434
ACACGTC57850.014.25231213
CGGGCAT24650.013.72051
CGGGCGC11700.013.56387415
CACGTCT64000.013.31436914
GACTGTC71000.013.3138964
TGCCGTC57500.013.19899948
CCGACTG60850.013.096742
CGAAGGG47400.012.73637648
Produced by FastQC (version 0.11.2)