FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_jes_117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_jes_117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13888921
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23450.024.71476244
TATGCCG25050.023.41135446
GTATGCC30650.019.35908545
TCGTATG32500.018.36378343
ATGCCGT32400.017.9939747
GCACACG35900.017.29651811
CACACGT39050.015.9015112
ACGTCTG39350.015.86920315
CCGACTG42250.015.0248742
CGCGTAT10850.014.9488671
ACACGTC42500.014.61073413
TGCCGTC42300.014.19033548
CTCGTAT38800.014.13780642
GACTGTC50050.013.9928764
CGGGCGC11600.013.97851915
GCCAGTA39800.013.959921
CGCGCGT21700.013.6766221
CGACTGT53400.013.5028293
GCCGTCT47700.013.09022449
CACGTCT49650.012.50739114
Produced by FastQC (version 0.11.2)