FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_jes_010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_jes_010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10111308
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19750.027.07363146
CGTATGC19800.026.83156844
GTATGCC22950.023.4490945
ATGCCGT24600.021.4554847
TCGTATG25650.021.25048443
GCACACG28850.020.56649411
CACACGT30150.019.56558812
ACGTCTG30400.019.29311615
ACACGTC32650.018.06754513
CGCGGGG4900.017.6089821
TGCCGTC31450.016.67265948
AGCACAC40200.015.53202210
CACGTCT39000.015.39190414
CACAGTG38350.015.38374433
GTCACAC37200.015.30300229
CTCGTAT29050.014.96398142
GCCGTCT35400.014.617393549
CACACAG39600.014.54979631
CGTCTGA43350.013.60944816
ACACAGT44100.013.06505932
Produced by FastQC (version 0.11.2)