FastQCFastQC Report
Wed 21 Jun 2017
HMNGTBGX2_n01_jes_009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNGTBGX2_n01_jes_009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12045782
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21250.030.35739746
CGTATGC22000.029.63646544
GTATGCC26300.024.65948145
ATGCCGT28850.022.83869247
ACTGACC28500.022.75802232
GCACACG31550.022.30553611
TCGTATG29600.021.9107443
CACACGT34250.020.44667612
ACGTCTG34400.019.8584115
CTCGTAT33750.018.29719742
ACACGTC37800.018.2528613
TGCCGTC38550.017.27096448
CACTGAC40750.016.424731
CACGTCT44200.015.68900214
AGCACAC48200.015.38768610
GCCGTCT46000.014.54887349
TCTCGTA45100.013.92316641
CGTCTGA53850.013.26246416
CGGGCGC7300.013.23344515
AGTCACT51950.013.21577628
Produced by FastQC (version 0.11.2)