FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505371
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN292410.2783433350426177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA31550.075.1216052
CACGCAC35550.066.94013
TAACAAG231500.060.396755
TAGTAAC233750.059.853222
CGCACTT53700.048.2414135
ACGCACT60300.047.3372084
GTAACAA298100.046.8640984
GTAGTAA341650.042.5132261
GTCTCGC10600.041.911921
ACAAGAG351850.039.601997
AACAAGA369250.037.9048776
CTCGCTC12200.036.5367473
CGAGCTA4200.034.2667125
AGTAACA425000.033.3253973
TCTCGGG16250.031.564999145
CAAGAGC371300.030.6038198
CAAGAGG92600.029.161778
AAACACG144600.028.3660165
CGTGCAC12400.027.8302783
ACACGAG107750.026.6654687
Produced by FastQC (version 0.11.5)