FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_P2_POSCONT_HA_PB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_P2_POSCONT_HA_PB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523231
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT72150.2859429041574077No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT66180.2622827636470858No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG64740.256575795081782No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC56910.22554415350794277No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG54260.21504174607873797No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA49500.19617704443231715No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG45790.18147367403143033No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT45170.17901650701025787No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG44800.17755013314278398No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT44210.17521186130005537No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT43170.1710901617806693No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA42810.16966341963934337No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA42510.16847446785490508No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT36340.14402169282162436No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT35860.14211936996652308No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT34320.13601608413973987No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT33990.13470823717685776No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG31700.12563257188897886No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT31160.12349245867698995No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT30970.12273945588017901No Hit
GTATAAGTTTGGTCCCCCATCTGATACCAATAGTCCTGCCCTTGATTGGG29700.11770622665939028No Hit
GATATACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGA29050.11513016445977399No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT28410.11259373398630565No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT28200.11176146773719886No Hit
CATAGTGAATGCACCAAATCATGAGGGAATACAAGCAGGAGTGGATAGAT27880.11049325250046468No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT27520.10906651035913874No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC27340.10835313928847577No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT27330.10831350756232783No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT26500.10502407429204856No Hit
GATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA26240.10399364941220206No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC26010.10308211971079936No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA26000.10304248798465143No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG25740.10201206310480491No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC25450.10086274304651457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGG1051.1095835E-955.23765145
ACTGATG16400.052.1642158
TCGGACA1405.456968E-1251.7852944
TTCGGAC1405.456968E-1251.7852943
TACTGAT16550.051.6914257
CATATAC16450.051.5649343
TCCTCCC2350.046.276222145
CGCTAGC1109.968062E-846.135993
TATACTG36900.045.5822875
TGGGTCC803.736459E-545.312138145
GTCGGCC1151.4138277E-744.13008145
CTTCTAA25500.044.068271
CGGACAT1652.910383E-1143.9390375
TTCTAAT27550.041.0523032
TAGATGG27100.040.9314048
TTATACT21100.039.51394
GCCGCTA1303.701589E-739.0381471
CAAGGCC1303.701589E-739.038147145
CCTTATA22750.036.9667362
GTATATG22500.036.7330361
Produced by FastQC (version 0.11.5)