FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612154
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCA32550.031.4053612
GTGCACC29300.026.7234044
CGTGCAC31750.026.716393
GATTGGG26600.026.1652722
CGTGCTC84800.024.5370123
TAGCGCT6800.024.5219257
ACAGCTC96650.023.8539833
ACGTGCT118300.023.8396852
TATACTG20700.023.8162965
GTAGCGC7100.023.4857866
GTGCTCC49050.023.2057844
TAACAAG571050.022.8652115
GTAACAA582900.022.4376894
AGCTGTT70500.022.4183182
TGCACCG37350.022.3225045
TAGTAAC594900.022.204452
AGTAACA597850.022.1433943
AACAAGT72800.022.1083986
GGATTGG34550.022.0331671
GAGCATC8250.021.9695784
Produced by FastQC (version 0.11.5)